Figure 1

Detection of three mutational events in exon 3 of PRSS1 resulting in a disruption of the R122 primary autolysis site of human cationic trypsinogen. Left panel: denaturing high performance liquid chromatography (DHPLC) profile of the mutant compared with the wild type sequence. Right panel: direct DNA sequencing of independently amplified polymerase chain reaction products. Mutations are indicated by arrows. A: c.365G>A(CGC>CAC; R122H); B: c.365~366GC>AT (CGC>CAT; R122H); C: c.364C>T (CGC>TGC; R122C). Refer to Table 1 for a summary of these mutational events.