Figure 5

Single TAS regions bounding the 81-MAC locus. Three pairs of display lanes are shown. Display reactions were performed from 7A-anchored products and from verification, tel-anchored templates. Left Arm-distal TAS region: displays and verifications are shown representing the O. trifallax 310 homozygote and O. fallax 3.5. The latter products show three bands, representing the strain's three alleles vA, vB, and vC (not shown, see text). Specific oligomers used: Extend = -123-; P1 = -387-; P2 primer = -903-; Display = LA4. Right Arm-distal TAS region of O. fallax 3.5, specific oligomers used: Extend = RA1.1; P1 = 3239; P2 primer = -894; Display = 247+.