Figure 3

Immunoblot with anti-phosphoglycerate mutase antiserum. Pretreatment was in the resting cell condition with glucose for 70 min at 23°C (left hand four extracts) or 37°C (right hand four extracts). The middle lane is a sample of purified phosphoglycerate mutase. Both wild type UBR1 and mutant ubr1 backgrounds were used.