Figure 1
From: Instability of the insertional mutation in Cftr TgH(neoim)Hgucystic fibrosis mouse model
Generation of the Cftr TgH(neoim)Hgu mouse model. a) Insertional disruption of the murine Cftr gene and predicted gene structure as described by Dorin et al. (1992). Abbreviations: S, Sal I; H, Hind III; X, Xba I. Map position of the CFneo1 and CFneo2 products is also indicated. b) Genotype analysis of heterozygous cf/+ matings. The probe 1.2H issued in order to identify via Southern hybridisation the congenic mice which carry the insertion. DNAs were digested with Xba I+Sal I and probed with 1.2H. The upper hybridising fragment of 6.6 Kb represents the wild type allele, the lower 5 Kb fragment is diagnostic for the insertional mutation.