Table 4 Primer sequences used for primer walking spanning the entire region from Cftr exon 9 to Cftr intron 10. Location based on the Genome Database Cftr sequence (AF 162137)
From: Instability of the insertional mutation in Cftr TgH(neoim)Hgucystic fibrosis mouse model
Primer name | Location | Sequence 5'-3' | Product Size |
|---|---|---|---|
NCEx9I9-A | 197720- 197742 | TTT GGG GAA TTA CTG GAG AAA G | 419 bp |
NCEx9I9-B | 198138- 198117 | AGC TCG CTG ATA GGT TAT CCA | |
NC10-A | 198002-198023 | CCC CTC CTC ACT TCC ATT AAA | 400 bp |
NC10-B | 198402-198381 | TTT AAG GCT CAG GGC TAA TTG | |
NC11-A | 198376- 198396 | TTC CAC AAT TAG CCC TGA GC | 649 bp |
NC11-B | 199024- 199001 | TGA AGG AAA TCA TTA CTG AAG CA | |
NC12-A | 199001- 199024 | TGC TTC AGT AAT GAT TTC CTT CA | 550 bp |
NC12-B | 199551- 199531 | TAT GGA TCC CCA CAG CAA GT | |
NC13-A | 199394- 199414 | CTC AGG GAT TGT CAC GGT TT | 563 bp |
NC13-B | 199966- 199946 | GCT TTG ATC TCT GGG AGC AC | |
NC14-A | 199741- 199763 | GAT CAC AGG AGC CTA GCA TAG A | 550 bp |
NC14-B | 200290- 200268 | TTC ACT TTA CAT CCT GGC TTC A | |
NC15-A | 200122- 200142 | ACT GGG AGA GGA TGC AAA AA | 575 bp |
NC15-B | 200696- 200676 | CCC AGT GTG AGA AGA TGC AC | |
NC16-A | 200572- 200592 | TGC TCC CAG AAA TCT TCA CC | 582 bp |
NC16-B | 201153- 201133 | AGT TGT CAG AAG GGA ACC CA | |
NC17-A | 201134- 201154 | TGG GTT CCC TTC TGA CAA CT | 582 bp |
NC17-B | 201715- 201695 | TTA GGT CCC CGT GCT TAC AC | |
NC19-A | 201739- 201759 | TAG GTG GAT CCA TAA CCC CA | 480 bp |
NC19-B | 202219- 202199 | GGA CAG AGA AGC AGG AGT GG | |
NC20-A | 202199- 202219 | CCA CTC CTG CTT CTC TGT CC | 487 bp |
NC20-B | 202686- 202666 | AAA GAA GAG CGA GCC CCT AC | |
NCI9Ex10-A | 202593- 202612 | CCA TAG CCC AAG AGC TTT CA | 413 bp |
NCI9Ex10-B | 203007-202987 | GTA CCC GGC ATA ATC CAA GA | |
NCEx10I10-A | 202986-203006 | TTC TTG GAT TAT GCC GGG TA | 403 bp |
NCEx10I10-B | 203387-203367 | TTT CCA GTT GGG GGT ACA CT | |
NC21-A | 203296- 203316 | GGG CTT CAA GGC CTA ATT CT | 479 bp |
NC21-B | 203775- 203755 | ATG TGA TCC AGA CTG GCC TA | |
NC22-A | 203654- 203674 | ATG CAT GGG GTG TGG TAC TT | 625 bp |
NC22-B | 204277- 204255 | TCC AAT GAT CTA CCT GTG TCC A |