Fig. 2

Phylogenetic analysis of grass Amy1 genes. Forty-three protein-coding sequences from 13 species were involved. The tree is rooted on midpoint and drawn to scale, with branch lengths measured in the number of substitutions per site. A discrete Gamma distribution was used to model evolutionary rate differences among sites [5 categories (+G, parameter = 0.4987)]. Two low-pI Amy2 genes, FN179389 [23] and Amy32b (GenBank accession no. X05166) were used as outgroup