Table 1 Checklist for experimentally validated miRNA-target interaction (MTI)
From: miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4
Item | Results |
|---|---|
1. miRNA gene name (Entrez ID) | miR-34a (100316602) |
2. Target gene name (Entrez ID) | ACSL4 (448980) |
3. Species name (Species ID) | Sus scrofa domesticus (9825) |
4. Genomic location of MTI Nucleotide sequence | 5'CACTGCC |
Location according to Ensembl | X:89761656-89761650 |
Location within a part of a gene | 1934-1940 (location within 3'UTR) |
Method for experimental validation | Luciferase reporter assay, qPCR, western blot |
Cell lines | 293 T cell lines, porcine primary intramuscular preadipocytes |
5. Sequence variants within MTI | None |
6. Associated phenotype | Adipogenesis |