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Table 4 Primers for 5′unknown sequences

From: Cloning and expression analysis of GATA1 gene in Carassius auratus red var

Primer name

Sequence (5′ → 3′)

Application

GATA1-SP1

CAGAGCAAGGCTGTGGAAGTCATTT

5′- Walking PCR amplification

GATA1-SP2

GTCCTGGTTTGGAGGTTGTTTGCC

 

GATA1-SP3

GCTTCCACCTTTGATAGAGGCTGA

 

GATA1-F3

ATGGCTGTAGTGCTCATTCATCGCT

verification

GATA1-R3

CAAGAGATTCACAACTATGACTGCG