Fig. 1

Isolation and identification of porcine BMMSCs and UCMSCs. A The fibroblast-like morphology of porcine MSCs. B FCM analysis of surface markers expressed on MSCs. Fluorescence in the range of M1 was considered an indicator that cells were recognized by the directed antibody. Autofluorescence intensity was less than 101; cells will fluorescence below this threshold were considered negative. C Osteogenic and adipogenic differentiation potential of porcine BMMSCs and UCMSCs. Calcium deposits in osteocytes and lipid droplets in adipocytes were stained red with Alizarin Red and Oil Red O, respectively. Scale bars, 50 μm