Table 3 Characterizations of six ORFs of ShVX isolate under study
S.No | ORF | Length (aa) | Components | Conserved motif Sequence | Indicative of |
|---|---|---|---|---|---|
1. | ORF1 | 1721 | Methyltransferase (40–336) | L-X6-LX6-L-X6-L | Methyl transferase |
Helicase (914–1145) | GxxGxGKS | Viral helicase | |||
RdRp (1267–1600) | SGX3TX3NTX18-37GDD | RNA-dependent RNA polymerase | |||
2. | ORF2 | 241 | Triple gene block 1 (TGB-1) | GVPGSGKS | RNA helicase |
3. | ORF3 | 103 | Triple gene block 2 (TGB-2) | Movement protein domain | Cell to cell movement |
4. | ORF4 | 380 | P42 | Â | Predicted to be involved in intracellular mobility |
5. | ORF5 | 262 | CP | Flexi_CP | Coat protein |
6. | ORF6 | 137 | Nucleic acid binding protein (NABP) | CFDCGAYLYDDHVCKRFTSRSNSDCLSVIH | Zinc finger motif. |
CCxH | Cysteine rich protein (CRP) |