Table 4 Results from gnomAD, I-Mutant2.0, Mupro, ConSurf and COACH
gnomAD | ||||
 Hub gene | SNP | Variation | Result | Effect |
  IL6 | rs2069849 | C-T | pass | *PLoF |
  IGF1 | rs35767 | A-C | not pass | - |
  TLR4 | rs1057317 | C-A | not pass | - |
  BGLAP | rs1800247 | T-C | pass with warning | - |
  BMP2 | rs2273073 | T-G | pass | *PLoF |
  TGFB1 | rs1800470 | G-A | pass | *PLoF |
  TP53 | rs1042522 | G-C | pass | *PLoF |
*PLoF - Predicted loss of function | ||||
 I-Mutant2.0 | ||||
  Hub gene | SNP | Variation | **DDG | Effect |
  IL6 | rs2069849 | F-L | -1.35 | Decrease stability |
  BMP2 | rs2273073 | S-A | 0.01 | Increase stability |
  TGFB1 | rs1800470 | P-L | -0.85 | Decrease stability |
  TP53 | rs1042522 | P-R | 0.38 | Increase stability |
**DDG - delta delta G, DDG < 0: Decrease Stability, DDG > 0: Increase Stability | ||||
 MUpro | ||||
  Hub gene | SNP | Variation | **DDG | Effect |
  IL6 | rs2069849 | F-L | -0.58605643 | Decrease stability |
  BMP2 | rs2273073 | S-A | -0.2650236 | Decrease stability |
  TGFB1 | rs1800470 | P-L | -0.050797501 | Decrease stability |
  TP53 | rs1042522 | P-R | 0.16475502 | Increase stability |
**DDG - delta delta G, DDG < 0: Decrease Stability, DDG > 0: Increase Stability | ||||
 ConSurf | ||||
  Hub gene | SNP | Position | Region |  |
  IL6 | rs2069849 | 201 | conserved region |  |
  BMP2 | rs2273073 | 37 | variable region |  |
  TGFB1 | rs1800470 | 10 | variable region |  |
 COACH | ||||
  Hub gene | SNP | Position | Prediction | Score |
  IL6 | rs2069849 | 201 | consensus binding residue | 0.02 |
  BMP2 | rs2273073 | 37 | not a binding residue | - |
  TGFB1 | rs1800470 | 10 | not a binding residue | - |
 YASARA FoldX | ||||
  Hub gene | SNP | Variation | Prediction | ΔΔG |
  IL6 | rs2069849 | F201L | Unstable | 1.936 |
ΔΔG < 0: Increase Stability, ΔΔG > 0: Decrease Stability | ||||