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Fig. 5 | BMC Genomic Data

Fig. 5

From: The amniote-conserved DNA-binding domain of CGGBP1 restricts cytosine methylation of transcription factor binding sites in proximal promoters to regulate gene expression

Fig. 5

CGGBP1 occupancy determines methylation restriction at target GC-rich TFBSs. (A) The abundance of the 45 motifs at which CGGBP1 restricts cytosine methylation were fetched from ChIP-seq data for full-length CGGBP1 (GSE187851) as well as the corresponding input. The occurrences were normalised to sequencing depths of input (red) and ChIP (green). The comparisons clearly showed a consistent enrichment of the motifs in CGGBP1 ChIP-seq (all motifs combined test p value < 0.0001). (B) To test if the N-term is capable of binding to these motifs, we compared the abundance of these motifs in input (red) and ChIP (green) for N-term. The comparisons show a strong enrichment in N-term ChIP-seq (all motifs combined test p value < 0.0001) showing that N-term is capable of and sufficient for occupancy at these motifs resulting in mitigation of cytosine methylation through steric hindrance

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BMC Genomic Data

ISSN: 2730-6844

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