Fig. 3

DEGs with an inversed expression profile in WT and PALS1 KO MDCK cells during cell polarization. A: 4-way Venn diagram illustrating overlaps of up- and downregulated DEGs of subsets A and B. The analyses revealed nine DEGs with a “inverse” expression pattern during the transition of nonconfluent (unpolarized) cell to polarized cell, meaning that these genes were upregulated in WT differentiation and downregulated in the KO differentiation process, or vice versa. B: Box plot of the six genes that were upregulated WT- and downregulated during differentiation of the KO cells. C: Box plot of the three genes that were upregulated in KO- and downregulated during differentiation of the WT cells. WT-nc; KO-nc: expression level of WT or KO cells grown under non-confluent conditions; WT-3D; KO-3D samples: expression levels of WT or KO cells grown as 3D cysts. Each dot represents the expression value of single samples of independent biological replicates