Fig. 7
Summary of DEGs of WT and PALS1 KO MDCK cell grown in 3D cyst cultures. A: Venn diagrams illustrating overlaps of total (upper diagram), upregulated (middle diagram) and downregulated DEGs of subsets C and D. The Venn diagrams of TOP20 up- and downregulated genes, overlapping in both subsets C and D. B and C: Volcano plots of DEGs of subsets C (B) and subset D (C), with annotated TOP20 up- and downregulated genes. y-axis: -log10, adj. P, x-axis: log2 fold change. DEGs are colored red and the TOP20 up- and down-regulated (by log-fold change) DEGs are labeled. Blue: genes with adj. P < 0.05. Yellow: genes with absolute log2-fold change > 1. D and E: Heatmap of TOP20 upregulated (D) and downregulated DEGs (E) showing the difference of relative gene expression levels in WT and KO cell grown 3D cyst (subset C) or in non-confluent monolayers (subset D)