Fig. 7
From: ERBB3-related gene PBX1 is associated with prognosis in patients with HER2-positive breast cancer
Interference with PBX1 inhibits proliferation and migration of HER2-positive BC cells and the process of EMT. A-C Detection of PBX1 interference levels by qRT-PCR (A) and western blot (B, C). D CCK8 detects the proliferation level of HCC1954 cells. E Edu staining (green) to observe the proliferation level of HCC1954 cells. DAPI (blue) restaining of cell nuclei. F-G wound healing (F) and transwell (G) assays for cell invasive migration levels. H-J western blot detection of migration and EMT-related protein expression levels in cells. BC, breast cancer; EMT, epithelial-mesenchymal transition. *p < 0.05, **p < 0.01, ***p < 0.001, and “ns” represents non-significant